Biotyping of Escherichia coli by a simple multiple-inoculation agar plate technique
نویسندگان
چکیده
منابع مشابه
Improved agar gradient-plate technique.
MIC of antimicrobial compounds, it is essential to have uniform microbial growth on the agar medium; otherwise, it is difficult to determine a sharply defined area of growth inhibition. The conventional agar gradient-plate method has been found to be unsatisfactory for determining the MIC of chemotherapeutic compounds or antibiotics which inhibit filamentous fungi because the latter frequently ...
متن کاملStudies with the Agar Cup-Plate Method: I. A Standardized Agar Cup-Plate Technique.
Official Circular 198 (Ruehle and Brewer, 1931) gives directions for performing the agar cup-plate test. However, in the limited literature dealing with the subject, no mention is made of the influence of varying the volume of agar, the amount of inoculum, the hydrogen ion concentration or other factors. In the course of other studies with the agar cup-plate method (Rose and Miller, 1939; Mille...
متن کاملA concise biotyping system for differentiating strains of Escherichia coli.
A six test biotyping system comprising fermentation of dulcitol, sorbose, raffinose, and 5 ketogluconate, motility and production of beta-haemolysis was used to obtain biotype profiles for 514 strains of Escherichia coli isolated from the urinary tract. This profile was suffixed to the API-20E code, recorded when the strains were originally identified. An expanded and reliable biotyping system ...
متن کاملSerotyping and biotyping of 160 Escherichia coli strains: comparative study.
One hundred and sixty Escherichia coli strains were serotyped and biotyped. Serotyping revealed 68 different types, with 25 strains not typable. Biotyping was possible in all strains but revealed 55 different types. One biotype could be subdivided into 35 different serotypes, indicating that for this biotype the series of biochemical and fermentation reactions could be extended for further diff...
متن کاملNew technique for biotyping.
An efficient technique for biotyping, using disposable trays instead of glass tubes and agar instead of liquid media, is described.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1979
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.10.3.275-278.1979